N-methyl-d-clucosamine complex of 4-methyluracil, method for producing and use thereof

ABSTRACT

The present invention relates to a novel substance, viz. Nmethyl-d-glucosamine complex of 4-methyluracil of the formula:   a process for producing same, and use thereof. The method for producing said substance consists in reacting 4methyluracil with an equimolar amount of N-methyl-d-glucosamine in an aqueous medium at a temperature of from 20* to 50*C, followed by isolation of the desired product from the solution. N-methyl-d-glucosamine complex of 4-methyluracil possesses a pharmacological activity and is useful in medicine as a medicated compound which is a cell-growth biostimulator increasing unspecific immunity and accelerating reparative regeneration of macroorganism tissues. The medicated compound has a high activity, sufficienty wide range of therapeutical applications, and a low toxicity. Said complex may be also useful as a growth stimulator for baking, nutrient, and other yeast.

United States Patent [191 Kulbakh et al.

[ 1 Oct. 14, 1975 [54] N-METHYL-D-GLUCOSAMINE COMPLEX OF 4-METHYLURACIL, METHOD FOR PRODUCING AND USE THEREOF [76] Inventors: Valter Osvaldovich Kulbakh,

Bukharestskaya ulitsa, 86, Korpus l, kv. 85; Inesa Vladimirovna Popova, Vasilievsky ostrov, 2 liniya, 25, kv.

' 27, both of Leningrad, U.S.S.R.

[22] Filed: Apr. 9, 1973 [21] Appl. No.: 349,670

[52] US. Cl 260/211 R; 260/211.5 R", 424/180 [51] Int. Cl. C07H 5/06 [58] Field of Search 260/21 1 R, 256.4 B

[56] References Cited UNITED STATES PATENTS 4/1967 Wakeman et al. 260/256.4 13 12/1971 Harrington et a1 260/21 1 R Primary ExaminerJohnnie R. Brown Assistant Examiner Cary B. Owens Attorney, Agent, or FirmWaters, Schwartz & Nissen [57] ABSTRACT The present invention relates to a novel substance, viz. N-methyl-d-glucosamine complex of 4-methyluracil of the formula:

H HO 0- N-cH, cHoH ,-cH,oH

a process for producing same, and use thereof.

The method for producing said substance consists in reacting 4-methyluracil with an equimolar amount of N-methyl-d-glucosamine in an aqueous medium at a temperature of from 20 to 50C, followed by isolation of the desired product from the solution.

Said complex may be also useful as a growth stimulator for baking, nutrient, and other yeast.

5 Claims, fNo Drawings l N-METHYL-D-CLUCOSAMINE COMPLEX or 4-METHYLURACIL, METHOD FOR PRODUCING AND USE THEREOF The present invention relates to novel compound,

Said novel compound comprises a white or yellowish water-soluble, hygroscopic, crystalline powder with a faint peculiar odor.

N-methyl-d-glucosamine complex of 4-methyluracil possesses pharmacological activity and is useful .in medicine as a medicated compound stimulating unspecific immunity and reparative regeneration of macroorganism tissues. Moreover, said compound may find application as a growth-stimulator for baking, nutrient, and other yeast.

According to the present invention, N-methyl-dglucosamine complex of 4-methyluracil is produced by reacting 4-methyluracil with an equimolar amount of N-methyl-d-glucosamine in an aqueous medium at a temperature of from 20 to 50C. The resulting desired product is isolated from a solution by crystallization on cooling or by sublimation drying with preliminary mechanical sterilization and freezing ofsaid solution.

As it has been mentioned hereinbefore, N-methyl-dglucosamine complex of 4-methyluracil is an active principleof a medicated compound comprising a cellgrowth stimulator. Thiamodicatod compound is used in such cases where it is necessary to stimulate unspecific immunity andreparative regeneration of tissues at ulceration including ulcer of the stomach and duodenum, sluggishly healing burning lesions wounds, bone fractures, radiorectitis colpitis, cystitis, colitis, and leucopeniae of different etiology. 5 v i The medicated compound of the present invention may be administered as a powder of N-methyl-dglucosamine complex of 4 -rnethyluracil for oral inges tion or local treatment (by powderi'ng). In the latter case, the medicated compound should be sterile, which is ensured in the process of producing'N-methyl-dglucosamine complex of 4-methyluracil' by mechanical sterilization of an aqueous solution of said substance, followed by freezing of the solution and sublimation drying.

Furthermore, for oral ingestion, the medicated compound may be used in the form of tablets containing 0.25 to 0.5 g of said active principle in combination with a pharmaceutical filler for tablets.

For intramuscular injections and intracavitary introduction, it is recommended to use a medicated compound comprising a 5% solution of the active principle in injection water. Such injection solution-may be prepareddirectly before use by dissolving a sterile powder of 'N-methyl-d-glucosamine complex of 4-methyluracil in ampulized water for injections.

Upon testing of the medicated compound of the present invention on animals, it has been found that N-methyl-d-glucosamine complex of 4-methyluracil stimulates complete restoration of leucocyte count in peripheral blood of rabbits treated with benzene, hinders the manifastation of nephrotoxic action of amphotericine B on rats, stimulates antiviral activity of serum and macrophages of abdominal cavity on mice.

The novel medicated compound is but slightly toxic. LD of said medicated compound for intravenous infusion is 2,120 mg per 1 kg of body weight on white mice. White mice show good endurance in respect of N-methyl-d-glucosamine complex of 4-methyluracil when administered hypodermally, intravenously, intraabdominally and per-orally in doses of up to 1.25 g/kg exceeding the therapeutical dose 500 times. When administered parenterally, the medicated compound exerts noirritatingeffect on tissues.

When the compound is used as tablets or powder, it is advisable to administer it to adults in doses of 0.25 to 0.5, g 3-4 times per day. A daily dose for children less than one year old is 0.03 g; l to 3 years old 0.05 g; 3 to 8 years 0.1 g; 8 to 12 years 0.2 g; 12 years and over as for adults. Such treatment course takes 10 to l5 days. Since the medicated compound of the presentinvention is low-toxic, the treatment course may be prolonged up to 1-1.5 months dependingon the disease development.

tion,,the latter should be preferably administered intramuscularly at a rate of 5 ml 2-3 times per day. The same solution in said dosage may be used for injecting round unhealable trophic ulcers and wounds, as well as for washing and rinsing in individually prescribed doses.

Sufficient water-solubility of N-methyl-dglucosamine complex of 4-methyluracil (50 to mg/ml which corresponds to a 5 to 7% solution) makes it possible to use it in medicine in small but efficient doses thus giving substantial economy of the medicated. compound. Therapeutical range of the preparation applications due to said property of the complex is broadened as compared to theprior-art medicated compounds of similar use.

The medicated compound of the present invention has another advantage residing in that unlike the priorart anabolizators pertaining to the class of steroids, it posesses no side effect.

Contra-indications against the use of the novel medicated compound are malignant diseases of bone marrow and lymphogranulomatosis.

The medicated compoundof the present invention i the powder form may be stored for long periods (for 1 year) in tightly sealed jars made of orange glass with gaskets of food grade rubber. The medicated compound intended for injections is stored in the form of a sterile powder in flasks in ampules packed in cartons. During storage, the medicated compound does not lose its bio-stimulating activity. f

The process for producing N-methyl-d-glucosamine complex of 4-methyluracil is preferentially realized in the following manner.

When the compound is used as a 5% injection solu- N'-m.ethyl-dg lucosamine is dissolved in water by stirring, the solution-is heated to a temperature of to C and, under vigorous stirring, an' equimolar amount of i -methyluracil is p'ortionw ise added thereto. The resulting solution is filtered for the separationof solid contaminants and then sterilized througha bacterial filter such as Millipore. The sterile solution of N-methyl-d-glucosamine complex of r 4- methyluracil thus obtained is then poured into sterilized flasks on a pipettin g machine, the solution is frozen i'n flasks at a temperature of from 30 to C and subjected tosu blimation drying. The flasks with driedpowder of N-methyl-d-glucosaminecomplex of 4-methyluracil are corked with sterile plugs made of food grade rubber and closed with aluminum cups with subsequent rolling thereof for the purpose of hermetic sealing. After checking the sterility of the prepared lot of the desired product, theflasks with the product are packed in cartons.

The medicated compounds in the form of a sterile powder in flasks and as a solution are intended for intrarnuscular injections and intracavitary' introduction. Moreover, the sterile powder may be used locally for treating wounds, burning lesions, and trophic ulcers by p'owdei'ing.

In addition to the above-described method of sublimatio'n drying, the resulting N-methyl-d-glucosamine complex of"4-'methylur'acil may be isolated from an aqueous solution by concentrating the solution with subsequent crystallization upon cooling. The precipitate of said complex is separated from the mother liquorby filtration and dried under vacuum. The resulting powd'er may be used per se for oral ingestion or may be tableted with a pharmaceutical filler for the same mode of introduction.

For better'understanding of the present invention the following' examples illustrating the production of N-me'thyl -d-glucosainin'e complex of l-methyluracil are given hereinbelow. w

EXAMPLE l 1.95 g(0.0l g-mol) of N-methyl-d-glucosamine were dissolved 'in 20 ml of water and the resulting solution was portion-wise added with"l.26 g (0.01 g-mol) of 4- methyl uracil uponheating the reaction mass to 50C onwat er bath. A clear solution of the resulting N-methyI-d-gIucosa'min'e complex of 4-methyluracil was con the solution turbidity, .said solution was filtered to remove mechanical contaminants. Thereafter, the solucentrated in-two steps and cooled to 10C. The resulting precipitate of said complex was separated'from the mother liquor byfiltration and dried under vacuumto give 2.6 g of N-methyLd-glucosamine complex of 4- methyluracil powder which corresponded to 81.5% of the theoretical. I

EXAMPLE 25 tion of N-methyl-d-glucosamine complex of 4- methyluracil was subjected to mechanical sterilization through a bacterial filter such as Millipore. A sterile solution of said comples was poured into sterilized flasks; the solution was frozen in the flasks at a temperature of 40C and subjected to sublimation drying. 31.5 g. of sterile powder of N-methyl-d-glucosamine complex of 4-methyluracil were thus obtained which corresponded to the yieldof 98% of the theoretical.

What is claimed is: i

1. N-methyl-d-glucosamine methyluracil of the formula:

complex of 4- 2. A method for producing N-methy'l-d-glucosamine complex of 4-methyluracil of the formula:

v H CH:

consisting essentially of reacting 4-methyluracil with an equimolar amount of N-methyl-d-glucosamine in an aqueous medium at a temperature of from 20 to 50C, followed by isolation of the desired product from. the solution. i

3. A method as claimed in claim 2, wherein the desired product is isolated from the solution by crystallization upon cooling. I

4. A method. as claimed in claim 2, wherein the desired product is isolated from the solution by sublimation drying with preliminary mechanical sterilization 

1. N-METHYL-D-GLUCOSAMINE COMPLEX OF 4-METHYLURACIL OF THE FORMULA:
 2. A method for producing N-methyl-d-glucosamine complex of 4-methyluracil of the formula:
 3. A method as claimed in claim 2, wherein the desired product is isolated from the solution by crystallization upon cooling.
 4. A method as claimed in claim 2, wherein the desired product is isolated from the solution by sublimation drying with preliminary mechanical sterilization and freezing of said solution.
 5. A method as claimed in claim 2, wherein the 4-methyluracil is present as 4-methyl-2,6-dioxypyrimidine in the basic solution. 